Table 1 List of vectors developed in this work

Fg vector

Destination vector for cloning and transformation into TSI locus 1

Spectinomycin

213464

pJET-LB-geneticin

Vector to amplify the LB-geneticin fragment for transformation into TSI locus 1

Ampicillin

213468

Fg-P_trpC-mCherry-T_trpC

Constitutive expression of a non-secreted version of mCherry

Spectinomycin

Fg-P_trpC-SP_osp24-mCherry-T_trpC

Constitutive expression of a secreted version of mCherry

Spectinomycin

Fg-P_Tri5-GFP-T_trpC

GFP expression controlled by the Tri5 promoter

Spectinomycin

Fg-P_FgEffector1-FgEffector1-GFP-T_trpC

Fgeffector1-GFP expression controlled by the Fgeffector1 promoter

Spectinomycin

Fg-P_trpC-GFP-T_trpC

Constitutive expression of a non-secreted version of GFP

Spectinomycin

Fg-P_osp24-osp24-T_osp24

osp24 expression under the osp24 promoter

Spectinomycin

pMU487

Vector to amplify the P_osp24-P_trpC-HyG_1-761 fragment for osp24 gene mutation

Ampicillin

pMU488

Vector to amplify the Hyg_296-1027-T_osp24 fragment for osp24 gene mutation

Ampicillin

pJET-P_trpC

Vector containing the trpC promoter for Golden Gate cloning

Ampicillin

213465

pJET-P_gpdA

Vector containing the gpdA promoter for Golden Gate cloning

Ampicillin

213466

pJET-T_trpC

Vector containing the trpC terminator for Golden Gate cloning

Ampicillin

213467