Fig. 5
Mitochondrial DNA integrity and respiratory capacity of strains expressing SjURA9. Top: sequencing coverage depth (average of non-overlapping 500 bp sliding window of the mitochondrial genome) obtained by short-read sequencing of mitochondrial DNA relative to the reference strain CEN.PK113-7D. Middle: phase contrast (PC) microscopy and fluorescence of nuclei and mitochondrial nucleoids of yeast cells stained with the DNA-specific fluorescent dye DAPI; scale bars represent 5 μm. Bottom: spot plate assays on glucose-containing synthetic medium with uracil (SMUD + ura) and ethanol-glycerol containing synthetic medium with uracil (SMEG + ura). SMUD + ura and SMEG + ura plates were incubated at 30 °C for 3 and 10 days respectively. Panels show data for the following yeast strains: A S. cerevisiae CENPK.113-7D (reference), B S. cerevisiae IME603 (multicopy ScURA1), C S. cerevisiae IMK242 (rip1Δ::kanMX), D S. cerevisiae IMI452 (ura1Δ::SjURA9, from IMX585), E S. cerevisiae IMI462 (ura1Δ::SjURA9, from IMX2600), F S. cerevisiae IMG008 (ura1Δ::SjURA9C265S), G S. cerevisiae IME571 (multicopy SjURA9) and, H S. cerevisiae IMS1206 (IME571 cured from its SjURA9 expression plasmid)