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Fig. 3 | Fungal Biology and Biotechnology

Fig. 3

From: Class-II dihydroorotate dehydrogenases from three phylogenetically distant fungi support anaerobic pyrimidine biosynthesis

Fig. 3

Complementation of uracil auxotrophy of S. cerevisiae ura1Δ strains by expressing mutated heterologous URA9 orthologs. The native ScURA1 gene of S. cerevisiae was replaced by URA9 orthologs from K. marxianus (Km), A. robustus (Ar) or Sch. japonicus (Sj) with single-nucleotide mutations affecting a specific serine or cysteine residue. Open bars indicate specific growth rates (μ) on synthetic medium without uracil (SMUD), closed bars indicate μ in uracil-supplemented synthetic medium (SMUD + ura). Cultures were grown aerobically or anaerobically as indicated in the Figure. Relevant genotypes of S. cerevisiae strains: IMG005; ura1Δ::KmURA9S263C, IMG007; ura1Δ::Arura9C168S, IMG008; ura1Δ::SjURA9C265S. Data represent the average from biological duplicates and mean deviation (Additional file 5: Table S1)

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