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Table 2 Primers used in this study

From: Expanding the toolbox: another auxotrophic marker for targeted gene integrations in Trichoderma reesei

Number

Name

Sequence (5′–3′)

A70

80820_5fwd-BspEI

TCCGGACTCTCAACCATGGCTTCAGAC

A71

80820_5rev-EcoRI

GAATTCTTATGGTTTGGTACTAGGTACTG

A72

80820_5fwd2-AflII

CTTAAGAGATGAAGTACTGCTATAAGCGC

A73

80820_5rev-SOE

CTTGACCTGATCCTTATGGTTTGGTACTAGGTACTG

A74

80820_3fwd-SOE

CCAAACCATAAGGATCAGGTCAAGGAGCACGATG

A75

80820_3rev-NsiI

ATGCATCTTTTCAGCACGGCACTTAC

A272

80820_5rev-MCS

GCTAGCTACCTTAGGCTGGAATTCCTGGGATCCTTATGGTTTGGTACTAGGTACTG

A273

P80820_fwd-MCS

GCTAGCCGTCATATGGGTCTGCAGTAGCACTGGACTTGATCACAG

A274

80820_3rev-ClaI

ATCGATCTTTTCAGCACGGCACTTAC

A294

Ppki_fwd-BamHI

GGATCCGACGGCCAGTGAATTCTCG

220

Tcbh2_rev_NheI

GCTAGCGCTATTAACGTTTGGAAAGC

A99

80820_5fwd2

GTTCTAAAGCCTCGTCGAGAG

A100

80820_3rev2

CCTTCAGCGTGAACGGACTAG

A63

pyrG_3fwd

ACATTGTGCCTGTCATTAAACG

A62

pyrG_5rev

AATGGGGTAGACAGGCAGAAC

233

Ppki_5rev

CAGCAGCCACGACAAAGC

45

cbh2terMF

CATCACAACCTCGTCTCCCTC

336

Ppki_Mrev

ATTAGGTGATGCTGCGCG

A772

sar1fw

TGGATCGTCAACTGGTTCTACGA

A773

sar1rev

GCATGTGTAGCAACGTGGTCTTT

A774

act1f

TGAGAGCGGTGGTATCCACG

A775

act1r

GGTACCACCAGACATGACAATGTTG

B069

eYFP_fwd_qPCR

TACAACTACAACAGCCACAACG

B070

eYFP_rev_qPCR

TTACTTGTACAGCTCGTCCATG

C12

cbh1_fwd_qPCR

GGCTAAAAGTACATAAGTTAATGCC

C13

cbh1_rev_qPCR

GACTTACATTTCAATATGGACCACT

212

Ppki_fwd-Kpn2I

TCCGGACGGCCAGTGAATTCTCGAG

214

Tcbh2_rev_PstI

CTGCAGGCCATCCAAAGAGCTCAACC