Fig. 3

Comparison of the RNP- and plasmid-based approach. a Editing efficiencies for single, double, triple and quadruple targeting. FnCpf1 was used to target all 4 genes simultaneously (‘4×’ pks4.2, alp1, snc1, ptf1) or only double or triple combinations thereof. Up to ten individual transformation experiments were performed for each setup. Note that no experiments for RNP_snc1 (1×), RNP_alp1 (1×) and (2×), plasmid_snc1 (1×), plasmid_alp1 (1×), (2×) and (4×) were done. Data for ptf1 were omitted in the diagram due to low targeting efficiency. b Pictures of transformation plates using different amounts of targets. Note that the targets for the RNP- and plasmid-based approach were identical except for 4× targeting. There, the targets were alp1, pks4.2, snc1, and ptf1 for the RNP approach and alp1, pks4.1, pks4.2 and snc1 for the plasmid approach