Fig. 2From: Practical guidance for the implementation of the CRISPR genome editing tool in filamentous fungiEditing efficiencies of four targets using the RNP approach. Three different nucleases were used to target 4 genes simultaneously (pks4.2, alp1, snc1, ptf1). Only the donor DNA of Δpks4.2 contained the selection marker amdS. Up to four individual transformation experiments were performed and 40–80 transformants were analyzed for each setupBack to article page