Fig. 1

Cas9-mediated gene deletion does not cause a disproportional increase in SNPs or indels in A. fumigatus. Segregation of genomic mutations into SNPs (b) and indels (a) revealed that the concentration of Cas9 was not positively associated with an increase in a specific subset of mutation. Shown are the average number of mutation events within each experimental group. Student’s t test assuming unequal variance was utilized for statistical comparison between the “no Cas9” and either the low (0.5 µg/µl) or high (1 µg/µl) Cas9 group