From: Efficient gene editing in Neurospora crassa with CRISPR technology
Primer name | Sequence 5′-3′ | Used for |
---|---|---|
β-tubulin-p F | TGCGACCAGGTTCAGGAGAGC | Genomic PCR (Figure 3e) |
actin F | GTCCCCGTCATCATGGTATC | qRT-PCR (Figure 4) |
actin R | CTTCTCCATGTCGTCCCAGT | qRT-PCR (Figure 4) |
clr-2 F | GCACCATCAATGTCGATACCTAC | |
clr-2 R1 | CATTGGCCACATGGTTGTTGACC | qPCR (Figure 3b, e) |
clr-2 R2 | CCATCACACCGAATCTTTCGTCT | qRT-PCR (Figure 4a) |
cbh-1 F | CTGCGTTGATGGTGCTGAGTAC | qRT-PCR (Figure 4b) |
cbh-1 R | GAGCTCGAAGCCCTGGTAGG | qRT-PCR (Figure 4b) |
gh5-1 F | CTCCTGCTAGCACCACCACTG | |
gh5-1 R1 | CAAGGCGCTCCATGGCGAAG | qPCR (Figure 3b, c) |
gh5-1 R2 | CTGCGGAGAGTCTGGATGGTG | qRT-PCR (Figure 4c) |
gh6-2 F | GCTCTGCCTGGAGCCAGTG | |
gh6-2 R1 | GTGGTGGTAACCTGAGCGCC | qPCR (Figure 3c) |
gh6-2 R2 | CTTGCTGGTGGTGCTAGAG | qRT-PCR (Figure 4d) |