Skip to main content

Table 2 Primers used in this study

From: Efficient gene editing in Neurospora crassa with CRISPR technology

Primer name

Sequence 5′-3′

Used for

β-tubulin-p F

TGCGACCAGGTTCAGGAGAGC

Genomic PCR (Figure 3e)

actin F

GTCCCCGTCATCATGGTATC

qRT-PCR (Figure 4)

actin R

CTTCTCCATGTCGTCCCAGT

qRT-PCR (Figure 4)

clr-2 F

GCACCATCAATGTCGATACCTAC

qPCR, qRT-PCR (Figures 3b; 4a)

clr-2 R1

CATTGGCCACATGGTTGTTGACC

qPCR (Figure 3b, e)

clr-2 R2

CCATCACACCGAATCTTTCGTCT

qRT-PCR (Figure 4a)

cbh-1 F

CTGCGTTGATGGTGCTGAGTAC

qRT-PCR (Figure 4b)

cbh-1 R

GAGCTCGAAGCCCTGGTAGG

qRT-PCR (Figure 4b)

gh5-1 F

CTCCTGCTAGCACCACCACTG

qPCR, qRT-PCR (Figures 3b, c; 4c)

gh5-1 R1

CAAGGCGCTCCATGGCGAAG

qPCR (Figure 3b, c)

gh5-1 R2

CTGCGGAGAGTCTGGATGGTG

qRT-PCR (Figure 4c)

gh6-2 F

GCTCTGCCTGGAGCCAGTG

qPCR, qRT-PCR (Figures 3c; 4d)

gh6-2 R1

GTGGTGGTAACCTGAGCGCC

qPCR (Figure 3c)

gh6-2 R2

CTTGCTGGTGGTGCTAGAG

qRT-PCR (Figure 4d)